SELECTIVE ELECTRON STAINING OF NUCLEIC ACIDS
نویسندگان
چکیده
منابع مشابه
Spirochete: Silver Staining for Nucleic Acids
Borrelia burgdorferi the Lyme disease spirochete was grown in modified Kelly medium and characterized by transmission and by scanning electron microscopy. Using silver staining procedures which preferentially bind to nuclear components of eukaryotic cells, signal could be detected by backscattered electron imaging throughout the length of the prokaryotic spirochete. Interestingly, however, the ...
متن کاملSelective "staining" for Electron Micrography
The physical basis of contrast and image formation in electron micrography is considered in relation to the possibility of recording selective chemical effects on cell components. A technology of selective microchemical analysis, equivalent to differential staining, is suggested as practicable in electron micrography. Electron pictures of bacteria after exposure to salts of heavy metals have sh...
متن کاملPreferential Staining of Nucleic Acid-containing Structures for Electron Microscopy
Oriented fibres of extracted nucleohistone were employed as test material in a study of satisfactory fixation, embedding, and staining methods for structures containing a high proportion of nucleic acid. Fixation in buffered osmium tetroxide solution at pH 6, containing 10(-2)M Ca(++), and embedding in Araldite enabled sections of the fibres to be cut in which the orientation was well preserved...
متن کاملIron as a Stain for Nucleic Acids in Electron Microscopy
An electron stain that would react with nucleic acids would obviously be a valuable addition to the techniques available for the electron microscopic investigation of cell structure. Information available from light microscopy (1-3) indicates that iron has a marked affinity for acidic residues as well as an excellent capacity for the penetration of biological materials. As a heavy metal, it was...
متن کاملSynthesis of Glycol Nucleic Acids Synthesis of Glycol Nucleic Acids
SYNTHESIS 2006, No. 4, pp 0645–0653xx.xx.2006 Advanced online publication: 25.01.2006 DOI: 10.1055/s-2006-926313; Art ID: M05505SS © Georg Thieme Verlag Stuttgart · New York Abstract: Starting from glycidol, the synthesis of dimethoxytritylated glycol nucleoside phosphoramidites of adenine (A), thymine (T), uracil (U), guanine (G), and cytosine (C) is reported. These phosphoramidites are the bu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1964
ISSN: 0022-1554,1551-5044
DOI: 10.1177/12.2.96